Using Mass Cytometry you have the ability to measure many parameters (up to 50 parameters) at once with a high level of data quality. Instead of fluorphores as used in regular flow cytometry, mass cytometry uses metal isotopes to identify your markers of interest. We have various large panels set up for convenient and easy use. Especially with our comprehensible analysis you can get the most out of your data.
Mass cytometry example
Identification of main leukocyte populations and CD45− cells in murine glioblastoma by manual gating and representation by viSNE. (A) Example gating of cell subsets. (B) viSNE embeddings color‐coded for lineage markers. (C) Populations gated in A are color‐coded according to the legend inset and displayed in the viSNE map.
Ref: Dusoswa et al. 2019
References
Dusoswa SA, Verhoeff J, Garcia-Vallejo JJ. OMIP-054: Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry. Cytometry A. 2019 Apr;95(4):422-426. doi: 10.1002/cyto.a.23725. Epub 2019 Jan 31. PMID: 30701669; PMCID: PMC6590190.
Verhoeff J, van Asten S, Kuijper L, van den Braber M, Amstalden-van Hove E, Haselberg R, Kalay H, Garcia-Vallejo JJ. A monodispersed metal-complexing peptide-based polymer for mass cytometry enabling spectral applications. N Biotechnol. 2024 Jul 25;81:33-42. doi: 10.1016/j.nbt.2024.03.002. Epub 2024 Mar 16. PMID: 38493996.
Rodriguez E, Zwart ES, Affandi AA, Verhoeff J, de Kok M, Boyd LNC, Meijer LL, Le Large TYS, Olesek K, Giovannetti E, García-Vallejo JJ, Mebius RE, van Kooyk Y, Kazemier G. In-depth immune profiling of peripheral blood mononuclear cells in patients with pancreatic ductal adenocarcinoma reveals discriminative immune subpopulations. Cancer Sci. 2024 Jul;115(7):2170-2183. doi: 10.1111/cas.16147. Epub 2024 Apr 30. PMID: 38686549; PMCID: PMC11247553.
Immune Monitoring Services 