Are you targeting the metabolic pathway to dampen inflammation? Or would like to find out if your therapy influences any metabolic pathway?
Ask our experts at the Immune-metabolic profiling platform for a fitting solution.
We established a broad array of techniques to phenotypically, functionally and metabolically profile immune cell subsets in a semi-high-throughput manner. This allows us to efficiently screen the effect of small molecules and genetic interventions on macrophages in vitro. Key targets are then further tested in in vivo disease models and patient material.
In-house techniques include:
- Extracellular flux analysis (Seahorse)
- Immunometabolic screens using Cytation multi-mode imaging reader
- Cytometry- and imaging based single-cell metabolic profiling (SCENITH, Met-Flow)
Extra cellular Flux assay
ECAR was assessed using the XF-96 Flux Analyzer (Seahorse Bioscience)
Ref Baardman et.al. Cell reports
Ref Verberk et.al. Cell rep methods
Multi mode imaging
Uptake of fluorescent nutrient analogs as assessed by multi-mode reader
Ref Verberk et.al. Cell rep methods
SCENITH Flow analysis
tSNE dimensionality reduction of naive, LPS ± IFNγ-, and IL-4-treated BMDMs
References
Verberk SGS, de Goede KE, Gorki FS, van Dierendonck XAMH, Argüello RJ, Van den Bossche J. An integrated toolbox to profile macrophage immunometabolism. Cell Rep Methods. 2022 Mar 28;2(4):100192. doi: 10.1016/j.crmeth.2022.100192. PMID: 35497494; PMCID: PMC9046227.
Artyomov MN, Van den Bossche J. Immunometabolism in the Single-Cell Era. Cell Metab. 2020 Nov 3;32(5):710-725. doi: 10.1016/j.cmet.2020.09.013. Epub 2020 Oct 6. PMID: 33027638; PMCID: PMC7660984.
Baardman J, Verberk SGS, van der Velden S, Gijbels MJJ, van Roomen CPPA, Sluimer JC, Broos JY, Griffith GR, Prange KHM, van Weeghel M, Lakbir S, Molenaar D, Meinster E, Neele AE, Kooij G, de Vries HE, Lutgens E, Wellen KE, de Winther MPJ, Van den Bossche J. Macrophage ATP citrate lyase deficiency stabilizes atherosclerotic plaques. Nat Commun. 2020 Dec 8;11(1):6296. doi: 10.1038/s41467-020-20141-z. PMID: 33293558; PMCID: PMC7722882.
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