Multiplex Immuno-histochemistry

Our multiplex Immunohistochemistry (IHC) panels are either fluorescent based (up to 8 colours) or imaging mass cytometry based (up to 40 colours) using the HyperionXTi.

Use our ‘know how’ in immune monitoring of the tumor microenvironment in your advantage. Having analyzed TMEs from lung, cervix, breast, prostate, brain, skin, oral cavity and esophagus we have a broad range of experience.

Tumor micro environment, showing lymphocyes such as B cells, T cells and regulatory T cells as well as their memory status. Multiplex immunohistochemistry using 7 colours.

This overlay of 7 markers show several lymphocytes

B cells, T cells and their memory status and T regulatory cells

Adjustable Lymphocyte panel

This overlay of 7 markers show several myeloid cells

Macrophages (M1 and M2 like), monocytes and mature Dendritic Cells

Adjustable Myeloid panel

Tumor micro environment, showing myeloid cells such as macrophages, Monocytes and dendritic cells. Multiplex immunohistochemistry using 7 colours.

References

Zwart ES, van Ee T, Affandi AJ, Boyd LNC, Rodriguez E, den Haan JMM, Farina A, van Grieken NCT, Meijer LL, van Kooyk Y, Mebius RE, Kazemier G. Spatial immune composition of tumor microenvironment in patients with pancreatic cancer. Cancer Immunol Immunother. 2023 Dec;72(12):4385-4397. doi: 10.1007/s00262-023-03573-6. Epub 2023 Nov 8. PMID: 37938368; PMCID: PMC10700423.

Zwart ES, van Ee T, Doppenberg D, Farina A, Wilmink JW, Versteijne E, Busch OR, Besselink MG, Meijer LL, van Kooyk Y, Mebius RE, Kazemier G. The immune microenvironment after neoadjuvant therapy compared to upfront surgery in patients with pancreatic cancer. J Cancer Res Clin Oncol. 2023 Nov;149(16):14731-14743. doi: 10.1007/s00432-023-05219-7. Epub 2023 Aug 17. PMID: 37587309; PMCID: PMC10603010.

Harmsen MJ, Arduç A, Bleeker MCG, Juffermans LJM, Griffioen AW, Jordanova ES, Huirne JAF. Increased Angiogenesis and Lymphangiogenesis in Adenomyosis Visualized by Multiplex Immunohistochemistry. Int J Mol Sci. 2022 Jul 29;23(15):8434. doi: 10.3390/ijms23158434. PMID: 35955568; PMCID: PMC9369277.

Goedegebuure RSA, Harrasser M, de Klerk LK, van Schooten TS, van Grieken NCT, Eken M, Grifhorst MS, Pocorni N, Jordanova ES, van Berge Henegouwen MI, Pouw RE, Verheul HMW, van der Vliet JJ, van Laarhoven HWM, Thijssen VLJL, Bass AJ, De Gruijl TD, Derks S. Pre-treatment tumor-infiltrating T cells influence response to neoadjuvant chemoradiotherapy in esophageal adenocarcinoma. Oncoimmunology. 2021 Aug 4;10(1):1954807. doi: 10.1080/2162402X.2021.1954807. PMID: 34377591; PMCID: PMC8344794.