Preventing or treating infectious diseases require a broad knowledge of the immune system and the immunomodulating properties of a vaccine or therapeutic.

From testing the immunogenic characteristics of a vaccine adjuvant or monitoring specific T and B cell responses, we have assays to get your questions answered.

• Proliferation assay
• Antigen specific T cell reactions (ELIspot, Proliferation assay, Flow Cytometry)
• MoDC generation
• Cytokine detection

References

Raaphorst H, Lougheed S, Saou L, van Kleef ND, Rensink I, Ten Brinke A, Freen-van Heeren JJ, Turksma AW. Exploring the dynamics of T-cell responses: a combined approach using EdU incorporation and proliferation dye dilution assay. Immunol Cell Biol. 2025 Feb;103(2):178-191. doi: 10.1111/imcb.12845. Epub 2024 Dec 30. PMID: 39740009.

Ten Brinke A, Marek-Trzonkowska N, Mansilla MJ, Turksma AW, Piekarska K, Iwaszkiewicz-Grześ D, Passerini L, Locafaro G, Puñet-Ortiz J, van Ham SM, Hernandez-Fuentes MP, Martínez-Cáceres EM, Gregori S. Monitoring T-Cell Responses in Translational Studies: Optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-Specific Responses. Front Immunol. 2017 Dec 21;8:1870. doi: 10.3389/fimmu.2017.01870. Erratum in: Front Immunol. 2018 Feb 22;9:343. doi: 10.3389/fimmu.2018.00343. PMID: 29312346; PMCID: PMC5742609.

Doeleman SE, Reijnders TDY, Joosten SCM, Schuurman AR, van Engelen TSR, Verhoeff J, Léopold V, Brands X, Haak BW, Prins JM, Kanglie MMNP, van den Berk IAH, Faber DR, Douma RA, Stoker J, Saris A, Garcia Vallejo JJ, Wiersinga WJ, van der Poll T. Lymphopenia is associated with broad host response aberrations in community-acquired pneumonia. J Infect. 2024 Apr;88(4):106131. doi: 10.1016/j.jinf.2024.106131. Epub 2024 Feb 29. PMID: 38431153.